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Product Info Summary
| SKU: | LS009132 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of potassium voltage-gated channel, KQT-like subfamily, member 4 (KCNQ4), transcript variant 1 |
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Product info
Product Name
KCNQ4 (NM_004700) Human Over-expression Lysate
SKU/Catalog Number
LS009132
Size
100 µg
Description
Transient overexpression lysate of potassium voltage-gated channel, KQT-like subfamily, member 4 (KCNQ4), transcript variant 1
Storage & Handling
Cite This Product
KCNQ4 (NM_004700) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS009132)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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KCNQ4 Human Over-expression Lysates NM_004700.
Western blot validation of overexpression lysate (LS009132) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNQ4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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KCNQ4 Human Over-expression Lysates NM_172163.
Western blot validation of overexpression lysate (LS009132) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with KCNQ4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for KCNQ4 (Source: Uniprot.org, NCBI)
Gene Name
KCNQ4
Full Name
Potassium voltage-gated channel subfamily KQT member 4
Weight
77.101kDa
Superfamily
potassium channel family
Alternative Names
DFNA2; DFNA2A; KQT-like 4; Kv7.4; potassium channel KQT-like 4; Potassium channel subunit alpha KvLQT4; potassium voltage-gated channel subfamily KQT member 4; potassium voltage-gated channel, KQT-like subfamily, member 4; Voltage-gated potassium channel subunit Kv7.4 KCNQ4 DFNA2, DFNA2A, KV7.4 potassium voltage-gated channel subfamily Q member 4 potassium voltage-gated channel subfamily KQT member 4|potassium channel KQT-like 4|potassium channel subunit alpha KvLQT4|potassium channel, voltage gated KQT-like subfamily Q, member 4|potassium voltage-gated channel, KQT-like subfamily, member 4
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on KCNQ4, check out the KCNQ4 Infographic
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In this infographic, you will see the following information for KCNQ4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For KCNQ4 (NM_004700) Human Over-expression Lysate (LS009132)
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No publications found for LS009132
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